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mariano garcia blanco  (Addgene inc)


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    Structured Review

    Addgene inc mariano garcia blanco
    Mariano Garcia Blanco, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mariano+garcia+blanco/pEF-BOS-T7-CA150+(Plasmid+%2321924)/pmc13000123-349-10-15
    Average 92 stars, based on 3 article reviews
    mariano garcia blanco - by Bioz Stars, 2026-11
    92/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Biallelic FRA10AC1 variants cause a neurodevelopmental disorder with growth retardation.
    Article Snippet: A SMU1 cDNA open reading frame clone (human) in a pcDNA3.1 + /C-(K)DYK vector was purchased from GenScript Biotech (#OHu13306D) and used to amplify the coding region of human SMU1 (NM_018225.3). .. Relevant restriction sites in primer sequences (NotI in the forward and XbaI in the reverse primer) allowed recombination of the PCR product in pMT2SM-HA vector by In-Fusion HD Cloning (TaKaRa) according to the manufacturer’s protocol. pGint, a reporter vector containing an enhanced GFP (EGFP) open reading frame divided into two exons by an artificial, constitutively spliced intron was a gift from Mariano Garcia-Blanco (Addgene plasmid # 24217; http://n2t.net/ addgene:24217; RRID: Addgene_24217).33 To eliminate a predicted canonical splice donor site within the EGFP open reading frame, the triplet GTG encoding valine 2 was deleted by using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s protocol. ..

    Cloning:

    Article Title: Biallelic FRA10AC1 variants cause a neurodevelopmental disorder with growth retardation.
    Article Snippet: A SMU1 cDNA open reading frame clone (human) in a pcDNA3.1 + /C-(K)DYK vector was purchased from GenScript Biotech (#OHu13306D) and used to amplify the coding region of human SMU1 (NM_018225.3). .. Relevant restriction sites in primer sequences (NotI in the forward and XbaI in the reverse primer) allowed recombination of the PCR product in pMT2SM-HA vector by In-Fusion HD Cloning (TaKaRa) according to the manufacturer’s protocol. pGint, a reporter vector containing an enhanced GFP (EGFP) open reading frame divided into two exons by an artificial, constitutively spliced intron was a gift from Mariano Garcia-Blanco (Addgene plasmid # 24217; http://n2t.net/ addgene:24217; RRID: Addgene_24217).33 To eliminate a predicted canonical splice donor site within the EGFP open reading frame, the triplet GTG encoding valine 2 was deleted by using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s protocol. ..

    Plasmid Preparation:

    Article Title: Biallelic FRA10AC1 variants cause a neurodevelopmental disorder with growth retardation.
    Article Snippet: A SMU1 cDNA open reading frame clone (human) in a pcDNA3.1 + /C-(K)DYK vector was purchased from GenScript Biotech (#OHu13306D) and used to amplify the coding region of human SMU1 (NM_018225.3). .. Relevant restriction sites in primer sequences (NotI in the forward and XbaI in the reverse primer) allowed recombination of the PCR product in pMT2SM-HA vector by In-Fusion HD Cloning (TaKaRa) according to the manufacturer’s protocol. pGint, a reporter vector containing an enhanced GFP (EGFP) open reading frame divided into two exons by an artificial, constitutively spliced intron was a gift from Mariano Garcia-Blanco (Addgene plasmid # 24217; http://n2t.net/ addgene:24217; RRID: Addgene_24217).33 To eliminate a predicted canonical splice donor site within the EGFP open reading frame, the triplet GTG encoding valine 2 was deleted by using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s protocol. ..

    Article Title: Huntingtin (HTT) interactome in regulation of DNA repair/remodeling and RNA processing pathways
    Article Snippet: .. The following plasmids were used: pEF-BOS-T7-CA150 was a gift from Mariano Garcia-Blanco (plasmid # 21924; Addgene; http://n2t.net/addgene:21924 ; RRID:Addgene_21924) ( ); pCMV5 BRGI-Flag was a gift from Joan Massague (plasmid # 19143; Addgene; http://n2t.net/addgene:19143 ; RRID: Addgene_19143) ( ); and pcDNA6-ARID1A was a gift from Ie-Ming Shih (plasmid # 39311; Addgene; http://n2t.net/addgene:39311 ; RRID:Addgene_39311) ( ). .. We have validated anti-phospho DNA-PKcs (S2056) antibody (#18192; Abcam) and total DNA-PKcs (E6U3A) antibody (#38168; Cell Signaling Technology) in our ISPNs using DNA-PKcs knockdown to confirm specificity ( ): ISPNs were transfected at a final concentration of 1.5uM with siRNA to DNA-PKcs (Santa Cruz Biotechnology) or with scrambled control siRNA.

    Mutagenesis:

    Article Title: Biallelic FRA10AC1 variants cause a neurodevelopmental disorder with growth retardation.
    Article Snippet: A SMU1 cDNA open reading frame clone (human) in a pcDNA3.1 + /C-(K)DYK vector was purchased from GenScript Biotech (#OHu13306D) and used to amplify the coding region of human SMU1 (NM_018225.3). .. Relevant restriction sites in primer sequences (NotI in the forward and XbaI in the reverse primer) allowed recombination of the PCR product in pMT2SM-HA vector by In-Fusion HD Cloning (TaKaRa) according to the manufacturer’s protocol. pGint, a reporter vector containing an enhanced GFP (EGFP) open reading frame divided into two exons by an artificial, constitutively spliced intron was a gift from Mariano Garcia-Blanco (Addgene plasmid # 24217; http://n2t.net/ addgene:24217; RRID: Addgene_24217).33 To eliminate a predicted canonical splice donor site within the EGFP open reading frame, the triplet GTG encoding valine 2 was deleted by using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s protocol. ..



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    92
    Addgene inc mariano garcia blanco
    Mariano Garcia Blanco, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mariano+garcia+blanco/pEF-BOS-T7-CA150+(Plasmid+%2321924)/pmc13000123-349-10-15
    Average 92 stars, based on 1 article reviews
    mariano garcia blanco - by Bioz Stars, 2026-11
    92/100 stars
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